Eighteen and thirty different isolates of terrestrial fungi and bacteria, respectively isolated from Egyptian environment (El-Fayoum, Egypt). All fungal isolates showed antioxidant activities in ethyl acetate and chloroform. Thirteen fungal isolates showed high antioxidant activities in case of using ethyl acetate as a solvent than using chloroform as solvents, five chloroform extracts showed high antioxidant activities than ethyl acetate extract. Fungal isolate No. 18(Penicillium lividum Westling)showed highest antioxidant activity (94.80℅).Fifteen bacterial isolates showed antioxidant activities in case of using ethyl acetate and chloroform, eleven isolates have significant antioxidant activities in case of using ethyl acetate as solvents. Only four chloroform extracts show antioxidant activities. Bacterial isolate No. 20(Bacillus azotoformans) showed highest antioxidant activity (69.27℅).