This study assesses the antinociceptive and anti-inflammatory activities of Galphimia glauca stem methanol extract in mice and rats. The methanol extract of Galphimia glauca stem was prepared and evaluated for in-vivo antinociceptive effect and anti-inflammatory properties in Swiss albino mice and Wistar albino rats. The dose range of 100, 200 and 400 mg/kg b.w was administered orally for one day for assessing antinociceptive and seven days for anti-inflammatory activity respectively. Further experimental studies were conducted for determining the involvement of central and peripheral receptor actions in the antinociceptive activity of the extract by prechallenging it with naloxone and acetic acid respectively. The in-vivo anti-inflammatory studies were conducted using carrageenan induced rat paw edema model and cotton pellet granuloma test. The LD50 of the extract was found to be > 2000 mg/kg b.w. The stem methanol extract at 400 mg/kg dose exhibited significant (P ≤ 0.001) and dose-dependent antinociceptive and anti-inflammatory activity. It also exhibited central and peripheral antinociceptive actions when treated with naloxone and acetic acid respectively. The results revealed that the stem methanol extract has more potential in terms of antinociceptive and anti-inflammatory properties