Arsenic contamination in ground water resource has become a serious worldwide problem. In India there are many such contamination cases reported in different parts of the country. Assam is one of such states in India which is under the threat of ground water arsenic contamination and many cases have been reported in its several districts. On this backdrop, the present study was carried out to assess the scenario of ground water quality of the North Karimganj Block of Southern Assam by collecting and analyzing 195 ground water samples during April-June, 2014 for As and Fe. The analytical report showed that, Sadarashi GP is the highest arsenic contaminated Gaon Panchayat compared to other three Gayon Panchayats in North Karimganj block of Karimganj district, Assam. It has also been noticed that, the Iron concentration in the 32 arsenic contaminated water samples are well above the permissible limit (0.3mg/l) prescribed by WHO. The results of our study indicated that the average concentration of arsenic in tube well water in contaminated villages ranged from (10.1 to 93.05) and that of Iron ranges from (2.404 to 45.34). Comparison of collected samples for both arsenic and iron with non-contaminated water shows p-value (<.01).