Taking wastewater treatment project in a natural rubber processing plant in Xishuangbanna for example, this paper introduces the operation of natural rubber wastewater treatment in A2O under bioaugmentation. The project adopted the wastewater treatment process of sand settling, anaerobic, oxygen deficit, aerobic, sedimentation and disinfection. At stable operation, indexes of PH, SS, COD, BOD5 and NH3-N in outlet water reached 7.43, 35mg/L, 40mg/L, 18mg/L and 0.27mg/L respectively. The quality of outlet water reached grade I discharge standard specified in Sewage Comprehensive Discharge Standard (GB8987—1996), and also reached urban reclaimed water reuse standard.