The present study was carried out to evaluate antitumor activity of polysaccharides from angelica and astragalus （AAP）in vitro. Inhibition rate, percentage of viability and IC50 were served as target to evaluate its antitumor activity on human tumor cell lines (Hela cell, HepG2 cell, A549 cell, SPCA-1 cell and BGC-823 cell) in vitro through MTT method. The results showed that AAP exhibited greater in vitro cytotoxicity on BGC-823 cell and Hela cell than on HepG-2, A549, SPCA-1 cells. And the inhibition rates of 5-FU on BGC-823 cell had no a significant difference from that of AAP at a concentration of 80 μg/mL. These results implied that AAP had an obvious antitumor activity in vitro.