In the present study, three plants Triticum aestivum, Piper betel and Carica papaya were used to evaluate the total phenolic and flavonoid content, metal chelating and antioxidant activity. Successive extractions were done by soxhelet apparatus using different solvent of different polarity petroleum ether, ethyl acetate, n-butanol. Aqueous extract was prepared by pulverization method. The results revealed that butanolic and aqueous extract showed higher total phenolic and flavonoid content in all three plants. N-butanol extract of Triticum aestivum and Piper betel showed high metal chelating and antioxidant activity as compared to Carica papaya. The results indicated that n-butanol extract of both plants have the potential to be a source of natural antioxidant and as a potent antihemolytic agent.