In the present investigation, the anti-nociceptive effect of methanolic extract of leaves of G. pentaphylla was studied using formalin test, radiant heat tail-flick test, hot plate test, writhing test & sensorimotor performance test at doses of 50-400 mg/kg, intra peritoneally. The extract produced a significant inhibition in the late phase of formalin induced pain. It did not exert any significant antinociceptive effect in tail flick test, but in the hot plate test, it significantly raised the pain threshold. In the writhing test it caused a significant decrease in the number of writhing in comparison with the control group (P<0.05). No significant change was observed in sensorimotor test. The results of pharmacological tests performed in the present study suggest that the methanolic extract of G. pentaphylla possess anti-nociceptive effect and these findings seem to justify the use of plant in traditional Indian medicine in the treatment of pain.