Urinary tract infections (UTIs) refers to the presence of microbial pathogens within the urinary tract. UTIs are also the most common nosocomially acquired infection which accounts for almost 40% of all nosocomial infections. The spectrum and susceptibility of these infections is different from that of community-acquired infections and they are more difficult to treat. This was a retrospective study conducted in Shri B M Patil Medical College, Bijapur, India over a period of 3 years. Patients with an episode of UTI that was not present in first 48 hours of admission, and became apparent after 48 hours of admission was diagnosed as nosocomial UTI, and were included in the study. Species were identified by conventional biochemical tests and were tested for antimicrobial susceptibility by the standard disc diffusion method recommended by the Clinical and Laboratory Standards Institute (CLSI). The prevalence of UTI was more among male patients. The majority of the isolates were from > 50 years age group. E. coli was the most predominant organism (43.3%), followed by Klebsiella spp. and Citrobacter spp. Piperacillintazobactum , amikacin, nitrofurantoin were the most active agents against the majority of Gram negative bacilli. E. coli, which was the commonest organism, isolated also showed similar sensitivity patterns. Piperacillin-tazobactum and linezolid were most effective drugs against Gram positive cocci. Sensitivity pattern of the pathogen should be ascertained before prescribing an antimicrobial agent for a meaningful therapy and to avoid fast emergence of resistant mutants. Appropriate usage of antimicrobials should be implemented by formulating hospital antibiotic policy through a close collaboration between clinicians and microbiologists.