Antibiotic resistance is a global public health threat, but nowhere is it as stark as in Indonesia. Research related to antimicrobial resistance and its mechanism to the existing health care situation in each country is a big challenge. This research explains about antimicrobial resistance in Salmonella enterica serovar Typhi mediated by efflux pump in Makassar Sulawesi Selatan using disk diffusion method which added efflux pump inhibitor (CCCP) 100 μM. From 20 selected patients, we found 14 isolates with characteristic: 11 isolates are MDR and 5 isolates sensitive to drug and effected to CCCP. Eight isolates resistant to chloramphenicol; 11 isolates are resistant to cotrimoxazole; 3 isolates each are resistant to tetracycline, amoxicillin and ciprofloxacin. In conclusion, while antimicrobial resistance was mediated by efflux pump in Makassar, multiple resistance was most often acquired.