A microbial infection always threatens to human being through its development on living and nonliving materials and imposes detrimental impact on textiles and user. The present investigation aims at developing an eco friendly natural antimicrobial finish on first aid dressing based cotton bandage treated with N-N-dimethylamine Nethylamine chitosan. The extensive study was conducted to assess the antimicrobial effectiveness of N-Ndimethylamine N-ethylamine chitosan by employing standard test methods. The finishing showed excellent potential against microorganisms under test and can be used for hygienic textile products.