The present study was conducted to determine antimicrobial and antioxidant activity of leaf and flower extract of Hypericum mysorense (Hypericaceae). Antibacterial and antifungal activity was determined by Agar well diffusion and Poisoned food technique respectively. Leaf extract showed high inhibitory activity against bacteria and fungi when compared to flower extract. Gram positive bacteria showed higher susceptibility to extracts when compared to Gram negative bacteria. Leaf extract inhibited C. capsici to high extent while flower extract inhibited B. sorokiniana to higher extent. Radical scavenging efficacy, as evaluated by DPPH scavenging assay, was high in case of flower extract when compared to leaf extract. The content of total phenolics was high in flower extract than leaf extract. A direct correlation was observed between phenolic content and radical scavenging efficacy of extracts. The plant appears to be promising source for development of agents with activity against pathogenic microorganisms and oxidative damage.