Indanone acetic acid is a non-heterocyclic fused ring of benzene with cyclopentananone moiety with acetic acid side chain in 1-position. This specific moiety has been synthesised and condensed with various substituents like PABA, piperidine, morpholine, benzimidazole, piperazine, pyrazolone and hydrazine to get the desired product for animicrobial screening by zone of inhibition study and MIC values with respect to some gram positive and gram negative microbes and fungal strains with reference standard antibiotics. The satisfactory result has been found in the antimicrobial and antifungal screening.