Co(II), Ni(II), Cu(II), and Zn(II) complexes has been prepared by the reaction of isatin with bromo and nitro derivative of aminophenol. The compounds were characterized by using various analytical techniques and spectroscopic studies like IR, UV-Vis, NMR and ESR which suggested octahedral type of geometry for the complexes. In this paper we have study the biological evaluation of these complexes. The compounds were screened for in vitro antimicrobial activity against gram positive bacteria viz Bacillus subtilis, Micrococcus luteus , gram negative bacteria- Pseudomonas aeruginosa, Pseudomonas mendocina and fungi Verticillum dahlia, Cladosporium herbarium, Trichophyton soudanense .The agar plate disc diffusion method was used for the evaluation of biological activity. The biological data revealed that transition metal complexes were more active than their Schiff base ligands.