In the present investigation the antimicrobial activity of silver nano-particle synthesis from Aspergillus sp. was investigated against common oral pathogen. , yeast like Candida glabrata, Candida albicans, and bacteria like Pseudomonas aeruginosa, Streptococcus mutans . In these tests, Muller Hinton agar plates were used and silver nanoparticles of various concentrations were supplemented . It was observed that yeast were inhibited at the low concentration of silver nano-particles, where as the growth-inhibitory effects of bacteria were inhibited at mild. The results indicate that the silver nanoparticles might be exploited as medical devices for the treatment of several infectious diseases caused by these organisms.