The Scorpion fish, Scorpaenopsis venosa collected from Kanyakumari coast, Tamil Nadu, West Coast of India was identified based on the morphological characters. The antibacterial assay with crude extracts and purified fractions of venom gland and gonad extract was carried out with six bacterial strains viz., Pseudomonas sp. Staphylococcus aureus, Vibrio harveyi, V. parahaemolyticus, Proteaus sp., and Escherichia coli. Antagonistic activity was noted against most of the bacterial species. The crude and fractions of gland and gonad, was potent against V. harveyi. The antifungal assay was carried out with three fungal strains viz., Rhizoctonia solani, Pythium proliferum and Aspergillus niger. Venom gland fractions inhibited the growth of Rhizoctonia solani. Other two fungi were resistant to venom gland and gonads crude extracts and fractions. The FTIR analysis of both the samples showed various peak values aligned as halide, amine, ether and alcohol.