Streptomyces strains isolated from different agro-ecological regions of soils was taken up to evaluate their antimicrobial potentials against the target organisms S. aureus, E. coli, P aeruginosa. In primary screening out of 3 strains of Streptomyces isolated from ten different soil samples of various regions, one showed antagonistic activity against all the three targeted organisms which was secondary screened against S.aureus, Bacillus, P aeruginosa, E coli, Klebsiella sp. and it was effective against all except Klebsiella sp. The strain was found to be the yellow pigmented Streptomyces isolated from Ennore region possessed strong antagonist activity against human pathogens which was found to be efficient bioactive compound.