The study screened the antimicrobial activity and phytochemical constituents of stem bark extract of Bryophyllum pinnatum. The antimicrobial activity was carried out using the method of Kirby Bauer, and the phytochemical screening was carried out using the method of Habourne (1973). The phytochemical screening revealed that saponin, flavonoid, alkoloid and Tannin were present while phenol was absent. The antimicrobial activity result showed that Staphylococcus aureus zones of inhibition for 125 mg, 250 mg, 500 mg, 100 mg and standard drugs 20 mg Ampiclox were 10.0 mm, 19.0 mm, 23.0 mm, 23.0 mm and 26.0 mm respectively. Streptococcus zones of inhibition at 250 mg, 500 mg, 1000 mg and 20 mg of Ampiclox standard were 12.0 mm, 26.0 mm, 27.0 mm and 27.0 mm respectively. Candida albicans zones of inhibition at 125 mg, 240 mg, 500 mg, 1000 mg and 20 mg Standard Ampiclox were 24.0 mm, 22.0 mm, 24.0 mm, 24.0 mm, 24.0 mm and 25.0 mm respectively. Escherichia coli and Streptococcus spp. was not inhibited by 125 mg but was inhibited at other concentrations. The Minimum inhibitory concentration for Staphylococcus aureus, Streptococcurs spp., Escherichia coli and candida albicans observed at 125 mg, 250 mg, 500 mg, 1000 mg and Ampiclox 20 mg showed that S. aureus has MIC at 1000 mg, E. coli at 20 mg Ampiclox, Staphylococcus spp. at 1000 mg and C. albicans at 20 mcg Ampiclox.