Piper attenuatum is a potent medicinal plant in the Indian systems of medicine. Piper attenuatum having Piperine, Piperlonguminine and other active constituents which are used as muscle relaxant, central nervous system depressant, in headache and as insecticide agent against Musca domestica. Piperine reduced the total body temperature and display analgesic, and anti-inflammatory activities. The aim of present study is to determine the Anti-Hyperlipidemic activity of Ethanolic seed extract of plant Piper attenuatum (Linn.) B. Ham. (Fam. Piperaceae). Piperine was the first amide to be isolated from Piper species. Ethanolic extracts of Piper attenuatum with a dose of 200 mg/kg exhibited significant Anti-Hyperlipidemic activity in Triton X-100 Induced Hyperlipidemia in rats (P<.05). It was found that Piperine & Piperlonguminine the active constituent of the plant was responsible for the Anti-Hyperlipidemic activity because this constituent have the ability to reduce the Total Cholesterol & Total Triglyceride level in rats. Fenofibrate was used as standard drug (65 mg/kg). The total time period of this study was one week.