Investigation of an in vivo application of chromium- amino acid complexes was conducted on nicotinamidestreptozotocin induced diabetic Wistar rats. The rats were divided into 9 groups each consist of 4 animals. Three groups are control (+) with chromium picolinate (CrPic), control (-)diabetic group (DM) and control non diabetic (non DM). Furthermore, three groups wereexamined on the effect of Formula A (Cr-glutamate by 1:3 ratio)at dose (100, 200 and 400 μg/day). In addition, the last 3 groups were studied on the effect of different formula of Cr (III)- glutamato made of Cr-glutamate by ratio 1:2 (200 μg/day), Cr-glycine (1:3) and Cr-cysteine(1:3) complexes. The blood glucose levels were measured before and after treatment. All formulas gave significant effect in lowering glucose level (p 0.05) compared to diabetic rats control group.