An ayurvedic formulation, Enterocin was tested for its antidiarrhoeal, antimotility and antisecretory activities. Antidiarrhoeal effect of Enterocin was evaluated in castor oil and magnesium sulphate induced diarrhoea, intestinal transit and intestinal secretion in mice at a dose of 2.5, 5, 10 ml/kg. Enterocin treated mice, significantly reduced the induction time of diarrhoea, weight of stools and number of stools in the diarrhoea induced by castor oil and magnesium sulphate. It has also produced antimotility and antisecretory activity in castor oil induced intestinal transit and intraluminal fluid accumulation in mice. Phytochemical analysis showed the presence of carbohydrates, steroids, triterpenoids, alkaloids, flavonoids and tannins as major constituents. These results suggest that Enterocin possesses antidiarrhoeal effect may be due to its antimotility and antisecretory effect. Antimotility and antisecretory effect of Enterocin may be due to the presence of different phytochemicals.