Based on the ethno pharmacological evidence, the present study was performed to investigate the anticancer activity of chloroform extract of aerial parts of Gomphrena globosa against Ehrlich Ascites Carcinoma (EAC) induced solid tumor. Acute toxicity study shows that, the extract was non-toxic up to 2000 mg / kg body wt. Administration of 200 or 400 mg / kg p.o. in mice, caused significant reduction in body weight, packed cell volume and viable cell count compared to the EAC control group, and restored the hematological and biochemical parameters towards normal value. Histological study indicated that the damaged tissue was recovered by extract and solid tumor volume was reduced significantly. Thus, the findings reveal a significant anti-cancer activity of the extract.