The aim of the present study was to isolate the actinobacteria from the faeces of domestic animals and to explore their biotechnological properties. A total of 87 actinobacterial cultures were isolated from different faeces of goat and chicken collected from various locations in Pudukkottai and Tiruchirappalli Districts, Tamil Nadu. Among the 87 actinobacterial isolates, 45 isolates were selected for the screening of antibacterial activity and extracellular digestive enzyme production based on distinct morphological features and growth rate. The actinobacterial isolates were varied one among them in their antibacterial activity and digestive enzyme producing abilities. Out of 45 isolates, 7 (15.6%) isolates showed multiple inhibitory activities against all the tested bacterial pathogens and 26 (57.8%) displayed excellent production of industrially important extracellular digestive enzymes.