Green nanotechnology process has emerged as a powerful approach for the synthesis of silver nanoparticles. This would offer numerous benefits including eco-friendliness and compatibility for biomedical applications. We used an environment friendly biosynthetic technique for the production of the AgNP’s. The reducing agents used to produce the nanoparticles were from aqueous extracts of Cycas circinalis, Ficus amplissima, Commelina benghalensis and Lippia nodiflora leaves. The antimicrobial activity of Ag nanoparticles was investigated against Escherichia coli, Klebsiella pneumoniae, Vibrio cholorae, Salmonella typhi and Shigella sonnei by disc diffusion method. These results suggest that Ag nanoparticles can be used as effective growth inhibitors in various microorganisms, making them applicable to diverse medical devices and antimicrobial control systems. AgNP’s are a promising candidate for development of future antibacterial therapies because of its wide spectrum of activity.