Microorganisms, of late have developed resistance to existing antibiotics, thereby leading to an increasing demand for new antibiotics. Since seaweeds offer a rich source of bioactive molecules, the present study was carried out to investigate its antibacterial potential. Two species of seaweeds namely, Sargassum ilicifolium and Kappaphycus alvarezii collected from different coastal regions of Rameshwaram (southeastern coast of Tamil Nadu, India) were used in the present study. For microbiological testing of the seaweed extracts, agar well diffusion method was used. The zone of inhibition was measured for all the different crude algal extracts against three strains of microorganisms namely, Escherichia coli, Salmonella sp. and Klebsiella sp. that cause diseases and disorders in human beings, animals and plants. Crude extracts prepared from chloroform, ethanol and methanol revealed a wide range of antibacterial activity against the test pathogens. Maximum inhibition was noticed with ethanol extracts in case of S. ilicifolium but all the three extracts showed varied results in case of K. alvarezii. However, no specific solvent exhibited activity against all the test organisms effectively. The overall antibacterial activity assessed from the above results indicates the presence of active constituents in the extractions of seaweeds which can be exploited for the production of lead molecules which are of use in pharmaceutical industry.