Faced with the methicilin resistant Staphilococcus aureus (MRSA) and the extended-spectrum beta-lactamase (ESBL) pandemic, this study reports the antibacterial activity of seven probiotics against two MRSA (Px1 and Px2) and six ESBL (Px3, Px4, Px5, Px6, Px7, and Px8) isolated from clinical patients. The mixed culture of probiotics was consisted of Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, Bifidobacterium bifidum, Bifidobacterium animalis, Lactobacillus plantarum, Streptococcus thermophilus. The probiotics mixture was performed by mixing equally each probiotic starter in MRS broth medium and then incubated at 35oC, 150 rpm for 48 hours. The anti bacterial activity of free cell supernatant (FCS) against each of the bacterial test was assayed by agar diffusion method by using hole as reservoir. The result showed that all the isolates exhibited very good inhibitory activity against target bacteria as indicated by the diameter of inhibition zone: ESBL (21-22 mm) and MRSA (19-20 mm). The potency ratio of the probiotics mixture was also evaluated against kanamycin, streptomycin, and ultra broad spectrum meropenem as standard.