Antibiotic resistance is becoming a global issue and is threatening the effective prevention and treatment of increasing infections caused by pathogenic microorganisms. Organotin(IV) compounds have been widely used in developing new antimicrobial agents. Two series of organotin(IV) dithiocarbamate namely methyl and ethylcyclohexyldithiocarbamate containing altogether six compounds were used to test their antibacterial activity using disc diffusion and microdilution methods. These compounds were tested against ESKAPE bacteria, which included Enterococcus raffinosus, Staphylococcus aureus, Klebsiella sp., Acinetobacter baumanni, Pseudomonas aeruginosa, and Enterobacter aerogenes. A microdilution test was carried out by using two-fold dilution with the highest concentration of40 mg/mL. The results showed that the compound3 had an antibacterial activity towards most of the bacteria tested with an inhibition range of 10–15 mm. The minimum inhibitory concentration (MIC) of compound 3 was same with the ampicillin, which was 2.5 mg/mL. All the compounds showed bacteriostatic effects as the minimum bactericidal concentration (MBC) values were higher than the MIC values. In conclusion, phenyltin(IV) methylcyclohexyldithiocarbamate has a potential to be developed as an antibacterial agent.