The present study was carried out to evaluate the In Vitro Antibacterial activity of crude extract of locally available plant Cassia auriculata flowers. The current study was performed to screen the phytochemicals that are present in C. Auriculata flowers. To prepare the extract, the shade-dried flowers of C. Auriculata were soaked in water, petroleum ether and methanol. The C. Auriculata flowers extract has several bioactive compounds.The TLC technique has been used to identified the possible compounds present in the methanol extract. The FT-IR spectral data shows functional groups of possible chemical compounds present in the methanol extract of C.Auriculata. The extract was subjected to Disc Diffusion Method to find out the biological activities with three different concentrations [50, 75,100 μl/ml].The methanol extract was only used for this study by using Disc Diffusion Method. Methanol extract exhibited significantly good anti-bacterial activity in a dose-dependent manner.