In this article, the antimicrobial effect of extracts from the pinus pollen was studied to provide the theoretical basis for application of the pinus pollen, and the research methods were used in course experiments to enhance students experiment skill. the pinus pollen extracts was extracted by alcohol abstraction method, and antimicrobial activities of extracts against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas stutzeri, Asparagillus niger and Saccharomyces cerevisiae were studied by inhibitory zone with filter paper and analysis of minimum inhibitory concentration. As a result, the extracts could inhibit B. subtilis, S. aureus, E. coli, P. stutzeri, A. niger and S. cerevisiae, and the extracts showed antibacterial effects against six bacterial stains: E. coli > B. subtilis > P. stutzeri > S. aureus > A. niger > S. cerevisiae. Extracts had the strongest antibacterial activity on E. coli and the weakest antibacterial action on S. cerevisiae, and inhibition rate increased gradually with increase of concentration. The antimicrobial experiments could improve students’ experiment skill. On the whole, the pinus pollen could inhibit the growth of microbes, and the antibacterial experiment of the pinus pollen could be used in biochemistry experiment and microorganism experiment.