The present work described a simple, precise, accurate and linear reverse phase HPLC method for determination of Phenoxymethy penicillin potassium from tablet formulations. The determination was carried out on a Hypersil, ODS, C-18 (30cm 4mm) (3 to 10mm) 5μ column using a mobile phase of water: acetonitrile: glacial acetic acid (500:500:5.75).The flow rate and run time were 1ml/min and 10 min respectively. The method was reproducible with theoretical plates and % RSD.