Journal of Chemical and Pharmaceutical Research (ISSN : 0975-7384)

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Original Articles: 2014 Vol: 6 Issue: 11

Analysis of Torsemide in bulk, dosage forms and dissolution samples using RP-HPLC-PDA method

Abstract

The aim of the present work was to develop and validate a simple, effective and economical HPLC method for the analysis of Torsemide in bulk, dosage forms and in dissolution samples. A Zorbax C18 reverse phase column (150 × 3.0mm, 3.5μm) and mobile phase containing 5mM ammonium acetate: acetonitrile (75:25 v/v) were used in isocratic mode. Quantification was achieved at 287nm. The retention time of Torsemide was 2.37 min and showed a good linearity in the concentration range of 10-50μg/mL with a correlation coefficient of 0.998. The validation parameters included specificity, linearity, and limit of detection, limit of quantification, precision, robustness and stability which fulfilled regulatory requirements in all the cases. The percent recoveries were in between 98-102 (RSD < 2). The method was successfully applied for the analysis of Torsemide in bulk, pharmaceutical dosage forms and in dissolution samples.

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