The aim of the present work is to develop and validate a simple, efficient, economical RP-HPLC method for the analysis of Rivastigmine (RVS) in drug in adhesive type transdermal patches and in transdermal permeation and/or release studies. An Inertsil-ODS-3 column (250 x 4.6mm, 5μm) with mobile phase containing 10mM ammonium acetate: methanol (35:65% v/v) at a flow rate of 1.0mL/min was used in isocratic mode and eluents were monitored at 217nm. The retention time of RVS was 5.32 min and showed a good linearity in the concentration range of 2- 10μg/mL with a correlation coefficient >0.999. The validation parameters like specificity, linearity, accuracy and limit of detection, limit of quantification, precision, robustness fulfilled regulatory requirements. The percent recoveries were ranged in between 98-102, (RSD < 2). The developed HPLC method was successfully used for the analysis of RVS in bulk and in house made transdermal patches and in transdermal permeation and/or release studies.