The bioactive components of leaves extract of Centella Asiatica have been evaluated by using HRLC-Ms techniques. This technique is easy, rapid and convenient to investigate the presence of medicinally active constituents from the successive methanolic leaves extract of Centella Asiatica. The remarkable advantages offered by this method are showing the presence of large number of constituents in the extract, few of them are likeValine, Triparanol, Butamben, ,Neuraminic acid, Aesculin , Esculetin, , Famciclovir ,Isocitretin, Rhoifoline, Gentiopicrin, Urocortisone , Pelargonic acid, Gabapentin, Ivermectin , sarmentoside ,Khivorin, Swietenine etc. and in negative chromatogram compounds like Xylocarpus , Losartan , Carbenicilline etc. The study is to focus the phytochemicals present in extract and their association with medicinal properties so as to find out the alternatives to the existing synthetic drugs.