Herbal drugs have been used since ancient times as medicines for the treatment of a range of diseases. In the present study n-butanol fraction and chromatographic elutes of n-butanol fraction (n-hexane, chloroform and chloroform: methanol (9:1) elutes) of ethanolic extract of Calotropis procera (Ait.) R. Br. leaves were evaluated for analgesic activity by tail immersion and hot plate method. The chloroform elute of n-butanol fraction significantly increased the time taken to withdraw the tail to the nociceptive stimuli (44.30 %) as well as increased the latency period of jumping or paw licking (69.62 %) at 100 mg/kg dose when compared with standard group animals. In conclusion, this study provides evidences for the analgesic activity of leaves of Calotropis procera which could partly contribute to its ethnomedical use.