Antibiogram is the periodic evaluation of antimicrobial susceptibility pattern of bacterial isolates in a local area. It helps clinicians in selecting antimicrobials for empirical therapy and monitors the development of resistance. This study is a move to determine the antimicrobial susceptibility pattern of E. coli from urine, exudate and blood samples. It is a retrospective review of in-patient culture results of urine, exudate and blood samples were investigated from January 2015 to December 2015. A total of 254 reports were obtained for analysis of antimicrobial susceptibility for 19 antibiotics. Out of the total 254 E. coli isolates, the highest number of positive E. coli isolates were found among urine samples (74%) followed by exudates (21%) and blood (5%). The 254 E. coli isolates studied were found to be highly sensitive to Meropenem (86%), Imipenem (86%), Amikacin (91%), Ertapenem (92%), Tigecycline (94%) and Colistin (98%), whereas they showed higher resistance rates to Ciprofloxacin (76%), Ceftriaxone (82%), Cefuroxime axetil (84%), Cefuroxime (85%), Ampicillin (89%) and Nalidixic acid (89%). E. coli isolates showed high rates of resistance to Ciprofloxacin, Ceftriaxone, Cefuroxime axetil, Cefuroxime, Ampicillin and Nalidixic acid and thus are inappropriate for in-patient treatment of E. coli in the study area. From this study it is suggested that Amikacin can be used as the drug of choice for empirical therapy and Cefaperazone/Sulbactam can be used as an alternative drug.