Acute respiratory infections (ARI) is an acute infection of any part of the respiratory tract and related structures including middle ear, and paranasal sinuses, and pleural cavity. This study was to determine the resistance in isolates of the pathogen of the patients with acute respiratory infections in clinics in Tasikmalaya against antibiotic amoxicillin and effectiveness of this antibiotic. Study was conducted the resistance test against pathogenic isolates of diseased patients ISPA. Results of the patient isolates shows that it is resistance to the antibiotic amoxicillin with the percentage of 70.45%.