The present study was designed to evaluate the efficacy of almond oil and diosmin extracted from lemon peels on the endothelial dysfunction in streptozotocin induced diabetic rats. The study was performed on 75 male white albino rats divided into 5 groups. Healthy rats served as control group, diabetic group, almond oil treated diabetic group, dimethylsulfoxide (DMSO) treated diabetic group, and diosmin treated diabetic group. Experimental diabetes was induced by single subcutaneous injection of 50mg/kg body weight streptozotocin. After two months blood samples were collected for assessment of serum triglycerides(TG), total, high density lipoprotein(HDL), and low density lipoprotein(LDL) cholesterol, fasting blood sugar (FBS), serum insulin, intercellular adhesion molecule-1 (ICAM- 1), nitrite and nitrate (NOx), plasma hydrogen peroxide (H2O2), glutathione peroxidase activity (GPX) and percent of DNA damage. Mean levels of total cholesterol, TG, LDL-cholesterol , FBS, percent of DNA damage and ICAM-1 were significantly low and HDL-cholesterol , insulin were significantly high in almond oil treated diabetic group compared to diosmin treated diabetic group. On the other hand no significant difference was observed between the two groups regarding NOx, GPX and H2O2. Supplementation with almond oil and diosmin exerted an anti atherogenic effect with amelioration of glycemic status along with decreased DNA damage in STZ induced diabetic rats, these effects are more pronounced in diabetic group treated almond oil .So it is recommended to use almond oil as a prophylactic measure for endothelial dysfunction in diabetics.