Adsorption of tartrazine by Annona muricata L seeds has been investigated. The experiment was done by batch system for examine the optimum conditions of dye adsorption. Effect pH, contact time, stirring rate, concentration and adsorbent dosage have been studied. Isotherm adsorption were measured experimentally. Maximum adsorption of dye was 23.6310 mg/g achieved at pH 2, contact time 120 minutes, stirring rate 100 rpm, initial concentration 600 mg/L and adsorbent dosage 0.1 g. Fourier Transform Infra-red spectroscopy was used for determination of functional group content in Annona muricata L. seeds. This spectrum indicated of hydroxyl group and carbonyl group as active site. The surface of Annona muricata L seed powder has porous and provide for dye uptake. Isotherm Langmuir and Freundlich model were indicated that the process adsorption was physic adsorption and adsorption process was pseudo-second order kinetic model with R² 0.982.