Aerobiology is a scientific and multi disciplinary approach focused on the transport of organisms and biologically significant materials. Fungi are remarkable for the ir antiquity, diversity, ubiquitous distribution an d longevity. Fungal spores have long been known as one of the important environmental bi o-particles causing dermatitis, respiratory and cardiac diseases along with allergic manifestation in human beings. In the present investigation, a new species of genera of fungus, Staphylotrichum wa s isolated and identified in October, 2013 as the d ominant aero mycoflora from aero mycoflora of Thaluk Hospital, P athannamthtta district, Kerala. The fungal culture was taxonomically identified and characteristic feature s were described.