RFID can give a unique digital identifier to real physical object, and bridges the physical world with the virtual digital world. Based on the analysis on our experience of RFID applications, some common key issues and requirements of RFID application are presented. A lightweight framework based on Web Service to support ubiquitous RFID application of medicine logistics is designed and implemented. This lightweight framework provides the integration of RFID technology and other holistic applications. Virtual object is used to map the real physical world object and the virtual digital object. Experiment result is given and some future research directions are discussed.