Diacetylmonoxime-3-amino-4-hydroxy benzoyl hydrazone (DMAHBH) is used as a novel chromogenic organic reagent for the determination of Lead (II) with specterophotometry. The novel chromogenic organic reagent Diacetylmonoxime-3-amino-4-hydroxy benzoyl hydrazone (DMAHBH) gave yellow color water soluble complex with Pb (II) in buffer (pH 4.0-5.0) medium. The complex shows maximum absorbance at 386 nm. The system obeyed beer’s law in the concentration range of 0.270-2.79mg/ml. The optimum Lead (II) concentration range for accurate determination as evaluated from Ringbom plot was 0.482-2.642 mg/ml. The molar absorptivity and Sandell’s sensitivity were 1.875 x 104 L.mol-1cm-1 and 0.0066 mg/cm2 respectively. The Lead (II) forms M:L (1:1) color complex with DMAHBH and stability constant of the complex was found to be. 8.8x106. Food, Soil and biological samples were analyzed for the determination of Lead (II) in the proposed method and obtained with good results.