Actinomycetes were also known as actinobacteria and there are Gram-positive, Spore forming, filamentous actinobacteria which usually contain about 57-75% of G+C content in their DNA. Actinomycetes are considered as a unique group of microorganism which locus between the true bacteria and the true fungi. Streptomyces genera occupy 50% of actinobacteria, in which 75% of commercially useful antibiotics produced by these genera. Pectinase, amylase, lipase, protease, cellulose and xylanase are industrially significant enzymes that are produced by the actinomycetes. About 22,500 bioactive compounds were produced by microbes, among this 17%, 38% and 45% were isolated from unicellular bacteria, fungi and actinomycetes respectively. The main aim of this review is to determine the various applications of endophytic actinomycetes.