It gives a new type of multi-channel temperature acquisition system that is equipped with Samsung S3C2440 and combined the Can bus communications technology. This system implements the acquisition, management and displaying printing of the multi-channel temperature data on the industry site. According to the control information, it collects multi-channel temperature, then passes the temperature signal to total controller by the Can bus. The hardware and program flow is also presented. Experiments shows, this system has the advantage of collection temperature precise, high reliability, and communication distance. So it can be widely used in various industrial controls.