A new precise and reliable first derivative spectroscopic method for Axitinib and Everolimus has been developed by using UV visible spectrophotometry in bulk and physical miture. The absorption maxima are 303 nm and 267.20 nm for Axitinib and Everolimus respectively in double distilled water. Different concentrations of drugs were scanned in the range of 200-400 nm, and the resulted graphs were converted into first order derivative spectra by using UV probe version software 2.43. These spectra of Axitinib and Eerolimus were overlaid and the Zero crossing (Zero absorbance) were determined, where Axitinib shows Zero absorbance at 213.40 nm but Everolimus shows absorbance at 213.40 nm. Also Everolimus shows Zero absorbance at 246.30 nm but Axitinib shows absorbance at 246.30 nm. These results correlates with the absorbance of physical mixture at 213.40 nm proportional to the concentration of Everolimus and the absorbance at 246.30 nm proportional to the concentration of Axitinib respectively. The linearity was found to be in the range of 5 to 35 µg/ml with correlation co-efficient of 1 for Axitinib and Everolimus independently.