A new flavonol, 2¢-hydroxy-7-methoxyflavonol (1) together with three known flavonoids, 7-O-methylglabranin (2), tephrowatsin C (3), and kaempferol-3-O-β-D-glucopyranoside (4), and a known dehydrorotenoid, dehydrodeguelin (5) were isolated from the flowers of Tephrosia tinctoria. The structure of the new as well as known compounds have been elucidated by extensive 2D NMR, ESI-TOFMS and chemical studies.