The one pot synthesis of 3-amino-4-imino-8-methoxy- (2H) Pyrazolo [3’,4’;4,5] pyrimido[2,1-b] benzothiazole and its 2-substituted derivatives is convenient over tradinational rout of synthesis of this organic compound. The novel compound are prepare by condensation of 3-cyno-4-imino- 2-methylthio-8-methoxy-4H-pyrimido [2,1-b] benzothiazole with 80% Hydrazine hydrate and other reagent. The structure of the compound was verified by 1H NMR and other spectroscopic techniques